It has been ten years since we published the first edition of this atlas (Paxinos et al., 1999 a, b) and much has been learned about the brain in the intervening period. We have attempted to incorporate this decade of advances into this new edition. The distribution of a chemical marker in the brain gives clues to regional organization. In this book we map the distribution of seven marker substances and, on the basis of these maps, we attempt to better define the postion of brain structures. This book features: • Fully labeled photographs of sections stained for parvalbumin, calbindin, calretinin, Nissl substance, neurofilament protein (SMI32 antibody), tyrosine hydroxylase, and nicotinamide adenine dinucleotide phosphate diaphorase (NADPH-d). • All sections are taken from a single brain sectioned in the coronal plane – seven out of every eight serial sections are displayed in labeled photographs in this book. • Thoroughly revised identification of structures with the benefit of the latest edition of The Rat Brain in Stereotaxic Coordinates (sixth edition, 2007). • A single volume (combining the previous two) in a convenient 14 x 11 inch hardback format.