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A Novel In Vitro Primary Culture Model of the Lower Motor Neuron-Neuromuscular Junction Circuit
chapterposted on 2023-05-22, 21:55 authored by Katherine SouthamKatherine Southam, Anna KingAnna King, Catherine BlizzardCatherine Blizzard, Graeme McCormackGraeme McCormack, Tracey DicksonTracey Dickson
Modelling the complex process of neuromuscular signalling is key to understanding not only normal circuit function but also importantly the mechanisms underpinning a range of degenerative diseases. Here, we describe a compartmented in vitro model of the lower motor neuron–neuromuscular junction circuit, incorporating primary spinal motor neurons, supporting glia and skeletal muscle. This culture model is designed to spatially mimic the unique anatomical and cellular interactions of this circuit in compartmented microfluidic devices, such that the glial cells are located with motor neuron cell bodies in the cell body chamber and motor neuron axons extend to a distal chamber containing skeletal muscle cells whilst simultaneously allowing targeted intervention.
Publication titleMicrofluidic and Compartmentalized Platforms for Neurobiological Research
Department/SchoolMenzies Institute for Medical Research
Place of publicationUnited States
Rights statementCopyright 2015 Springer Science+Business Media New York