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Chapter 18. Morphological Analysis of the Zebrafish Digestive System

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posted on 2023-05-22, 22:12 authored by Andrew TrotterAndrew Trotter, Parslow, AC, Heath, JK
The zebrafish provides an ideal model for the study of vertebrate organogenesis, including the formation of the digestive tract and its associated organs. Despite optical transparency of embryos, the internal position of the developing digestive system and its close juxtaposition with the yolk initially made morphological analysis relatively challenging, particularly during the first 3 d of development. However, methodologies have been successfully developed to address these problems and comprehensive morphologic analysis of the developing digestive system has now been achieved using a combination of light and fluorescence microscope approaches—including confocal analysis—to visualize wholemount and histological preparations of zebrafish embryos. Furthermore, the expanding number of antibodies that cross-react with zebrafish proteins and the generation of tissue-specific transgenic green fluorescent protein reporter lines that mark specific cell and tissue compartments have greatly enhanced our ability to successfully image the developing zebrafish digestive system.

History

Publication title

Zebrafish: Methods and Protocols

Volume

546

Editors

GJ Lieschke, AC Oates, K Kawakami

Pagination

289-315

ISBN

978-1-60327-976-5

Department/School

Institute for Marine and Antarctic Studies

Publisher

Humana Press

Extent

19

Rights statement

Copyright Humana Press 2009

Repository Status

  • Restricted

Socio-economic Objectives

Expanding knowledge in the biological sciences

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