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Quantifying kinetics of net ion fluxes from plant tissues by non-invasive microelectrode measuring MIFE technique

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posted on 2023-05-22, 13:39 authored by Sergey ShabalaSergey Shabala, Tracey Cuin, Svetlana ShabalaSvetlana Shabala, Ian NewmanIan Newman
Non-invasive microelectrode ion fl ux measuring (the MIFE system) allows concurrent quantification of net fluxes of several ions with high spatial (several μm) and temporal (ca 5 s) resolution. Over the last 10 years, the MIFE system has been widely used to study various aspects of salt stress signaling and adaptation in plants. This chapter summarizes some major findings in the area such as using MIFE for deciphering the specific and non-specific components of salinity stress, resolving the role of the plasma membrane H+-pump in salinity responses, proving K+ homeostasis as a key feature of salinity tolerance, and discovering the mechanisms behind the ameliorative effects of Ca2+ and other mitigating factors (such as polyamines or compatible solutes). The full protocols for microelectrode fabrication, calibration, and use are then given, and two basic routines for measuring net K+ and Na+ fluxes from salinity stressed roots are described in the context of plant screening for salt stress tolerance.

History

Publication title

Plant Salt Tolerance: Methods and Protocols

Editors

S Shabala and TA Cuin

Pagination

119-134

ISBN

978-1-61779-985-3

Department/School

Tasmanian Institute of Agriculture (TIA)

Publisher

Humana Press

Place of publication

New York

Extent

28

Rights statement

Copyright 2012 Springer Science+Business Media, LLC

Repository Status

  • Restricted

Socio-economic Objectives

Other plant production and plant primary products not elsewhere classified

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