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Control of the polymerization towards porous layer open tubular capillary columns
conference contributionposted on 2023-05-23, 17:33 authored by Knob, R, Michael BreadmoreMichael Breadmore, Rosanne Guijt, Petr, J, Miroslav MackaMiroslav Macka
A novel method for the preparation of monolithic porous layer open tubular (PLOT) columns in fused silica capillaries is presented. In the first experiments, light attenuation by a UV absorbing compound in the polymerization mixture was tested. In another approach, the capillary was first flushed by polymerization mixture followed by a solvent not containing the monomers. However, no useful results were obtained in both cases. When the polymerization mixture at the centre of the capillary was removed with an immiscible liquid (FC-770), a thin layer of polymer monolith was formed. The flushing with FC-770 leaves only a thin film of the polymerization mixture wetting the capillary wall. Subsequent UV initiated polymerization of this film results in PLOT columns with a thin layer of poly(glycidyl methacrylate-coethyleneglycol dimethacrylate) at the wall of a fused silica capillary.
Publication titleProceedings of CECE 2012
EditorsF Foret, J Křenková, A Guttman, K Klepárnik, P Boček.
Department/SchoolSchool of Natural Sciences
PublisherInstitute of Analytical Chemistry ASCR, v. v. i.
Place of publicationBrno, Czech Republic
Event title9th Internation Interdisciplinary Meeting on Bioanalysis (CECE 2012)
Event VenueBrno, Czech Republic
Date of Event (End Date)2012-12-02