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Identification of a cell-surface located protein with growth at low pH in Bifidobacterium animalis (syn. lactis) Strain Bb12

conference contribution
posted on 2023-05-24, 10:27 authored by Pepper, S, Margaret BritzMargaret Britz

Bifidobacterium animalis (syn. lactis) has been used as a starter culture in several manufactured product lines including yoghurts and anti-diarrhea tablets. As a starter culture it has been selected through a series of mutations and propogations to make it suitable for use in dairy products. This strain was studied to determine the affect of a sudden decrease in pH or continuous exposure to low pH.

Protein profiles of membrane and cell-surface associated fractions, for growth at both neutral pH (7.0) and mildly acidic conditions were essentially identical. At pH 5.0 an increase was noted in the density of a band around 33kOa. The same band was markedly increased during growth at pH 4.0. A 14 fold increase was observed in the relative amount of the protein present in cultures grown at pH 4.0.

Marked up-regulation of a protein of a protein of approximately 33kOa was a feature of 50S-PAGE gels of cells of Bifidobacterium animalis (syn. lactis) strain VUP Bb 12 grown under strongly acidic conditions.


Publication title

7th International Symposium on Industrial Microbiology & Technology


Tasmanian Institute of Agriculture (TIA)

Event title

In Honour of Progessor Arnold L Demain

Event Venue

Melbourne, Australia

Date of Event (Start Date)


Date of Event (End Date)


Repository Status

  • Restricted

Socio-economic Objectives

Dairy products not elsewhere classified

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