Microscale photolytic eluent generation for chromatography

This paper describes a new method for the generation of gradients for microscale chromatography based on the photolytic degradation of photosensitive reagents. An inert dissolved photochemical is placed in the eluent and irradiated by variable intensity LEDs to proportionately degrade the photochemical into simple chemical components, such as HCl, which can be used to create pH gradients which can be used to perform gradient separations of antipsychotics.