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Nanofluidic trap for DNA extraction from biological samples
conference contribution
posted on 2023-05-23, 12:27 authored by Shallan, AI, Rosanne Guijt, Michael BreadmoreMichael BreadmoreA new device seamlessly integrating various electrokinetic processes enables the analysis of short ssDNA (20 bases) directly from whole blood. An electrokinetic trap was integrated to achieve extraction, concentration, and desalting of ssDNA in 200 s followed by a 90 s electrophoretic separation. The trap consists of two sequentially coupled nanojunctions with different pore size and exhibits selective ion transport properties. Depletion forces near the nanojunctions and the apparent electromigration were tuned to enable the trapping and subsequent analysis of fluorescein labeled-ssDNA. The quantitation limit (S/N ratio = 10) was 12.5 nM using 40 μL of spiked whole blood.
History
Publication title
Proceedings of MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life SciencesPagination
1963-1965ISBN
978-097980648-3Department/School
School of Natural SciencesPublisher
Chemical and Biological Microsystems SocietyPlace of publication
Washington DC, USAEvent title
MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life SciencesEvent Venue
Gyeongyu, KoreaDate of Event (Start Date)
2015-10-25Date of Event (End Date)
2015-10-29Rights statement
Copyright 2015 Chemical and Biological Microsystems Society (CBMS)Repository Status
- Restricted