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Nanotube molecular probes: DNA hybridization using single walled carbon nanotubes as biomarkers
conference contribution
posted on 2023-05-23, 12:42 authored by Cao, C, Hong, S, Choi, J-B, Kim, Y-J, Baik, S, Hwang, E-S, Jung, H-J, Cha, C-YSingle walled carbon nanotubes (SWNTs) hold particular advantage as optical nano-bio markers because they exhibit strong Raman signals. Such signals do not blink or photobleach under prolonged excitation. Here, new single-stranded DNA conjugated SWNT probes were developed to locate a particular sequence of DNA within a complex genome. Chromosomal DNAs of human fibroblast and Escherichia coli (E.coli) were used as a target and a control, respectively. Southern blotting, which uses photostable Raman signals of nanotubes instead of fluorescent dyes, demonstrated excellent sensitivity and specificity of the probes. The results showed SWNTs' possibility of being generic nano-bio markers for precise detection of specific kinds of genes.
History
Publication title
Proceedings of IEEE SENSORS 2006Pagination
1272-1275ISSN
1930-0395Department/School
School of Natural SciencesPublisher
IEEEEvent title
IEEE SENSORS 2006Event Venue
Daegu, KoreaDate of Event (Start Date)
2006-10-22Date of Event (End Date)
2006-10-25Rights statement
Copyright 2006 IEEERepository Status
- Restricted