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Nanotube molecular probes: DNA hybridization using single walled carbon nanotubes as biomarkers

Version 2 2025-01-15, 01:13
Version 1 2023-05-23, 12:42
conference contribution
posted on 2025-01-15, 01:13 authored by C Cao, S Hong, J-B Choi, Y-J Kim, S Baik, E-S Hwang, H-J Jung, C-Y Cha
Single walled carbon nanotubes (SWNTs) hold particular advantage as optical nano-bio markers because they exhibit strong Raman signals. Such signals do not blink or photobleach under prolonged excitation. Here, new single-stranded DNA conjugated SWNT probes were developed to locate a particular sequence of DNA within a complex genome. Chromosomal DNAs of human fibroblast and Escherichia coli (E.coli) were used as a target and a control, respectively. Southern blotting, which uses photostable Raman signals of nanotubes instead of fluorescent dyes, demonstrated excellent sensitivity and specificity of the probes. The results showed SWNTs' possibility of being generic nano-bio markers for precise detection of specific kinds of genes.

History

Publication title

Proceedings of IEEE SENSORS 2006

Volume

302

Pagination

1272-1275

ISSN

1930-0395

Department/School

Biological Sciences

Publisher

IEEE

Publication status

  • Published

Event title

IEEE SENSORS 2006

Event Venue

Daegu, Korea

Date of Event (Start Date)

2006-10-22

Date of Event (End Date)

2006-10-25

Rights statement

Copyright 2006 IEEE

Socio-economic Objectives

280105 Expanding knowledge in the chemical sciences