Nanotube molecular probes: DNA hybridization using single walled carbon nanotubes as biomarkers
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conference contribution
posted on 2025-01-15, 01:13authored byC Cao, S Hong, J-B Choi, Y-J Kim, S Baik, E-S Hwang, H-J Jung, C-Y Cha
Single walled carbon nanotubes (SWNTs) hold particular advantage as optical nano-bio markers because they exhibit strong Raman signals. Such signals do not blink or photobleach under prolonged excitation. Here, new single-stranded DNA conjugated SWNT probes were developed to locate a particular sequence of DNA within a complex genome. Chromosomal DNAs of human fibroblast and Escherichia coli (E.coli) were used as a target and a control, respectively. Southern blotting, which uses photostable Raman signals of nanotubes instead of fluorescent dyes, demonstrated excellent sensitivity and specificity of the probes. The results showed SWNTs' possibility of being generic nano-bio markers for precise detection of specific kinds of genes.
History
Publication title
Proceedings of IEEE SENSORS 2006
Volume
302
Pagination
1272-1275
ISSN
1930-0395
Department/School
Biological Sciences
Publisher
IEEE
Publication status
Published
Event title
IEEE SENSORS 2006
Event Venue
Daegu, Korea
Date of Event (Start Date)
2006-10-22
Date of Event (End Date)
2006-10-25
Rights statement
Copyright 2006 IEEE
Socio-economic Objectives
280105 Expanding knowledge in the chemical sciences