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Stages of embryonic development from zygote to hatching in the viviparous fish, Gambusia holbrooki

conference contribution
posted on 2023-05-24, 18:22 authored by Seyed Ehsan MousaviSeyed Ehsan Mousavi, Jawahar PATILJawahar PATIL, Gary PurserGary Purser
Developmental staging is indispensable to study developmental pathways, genetics and evolution among taxa. Life-history stages have been described for few Poeciliid species, however, proposed classifications are not generally applicable due to divergent morphology among the different Poeciliid species. In Poeciliid, with placentation developmental strategy, embryos receive the maternal investment throughout gestation. Therefore, normal morpho-physiological characteristics are often compromised by applying previously employed in vitro culture and embryo fixation approaches. Here, we describe the early development of the embryonic process in a livebearing fish, Gambusia holbrooki, based on detailed morphological diagnostics and overlay cardiac rate in developing embryos under normal conditions. Light microscopy observations of live embryos obtained from gravid wild captured females, allowed assignment of the development process into seven periods: zygote, cleavage, blastula, gastrula, segmentation, Pharyngula, and hatching. Thirty-seven stages were determined and assigned within each of these periods applying a numbered staging system. The novelty of the present study is the real-time, in vivo quantification of heart rhythm variations as a marker for embryonic staging from early gastrulation to late hatching. Moreover, at least one morphologic landmark for each of the embryonic stages was defined to maximize the accuracy of staging.

History

Publication title

Australian Society for Fish Biology Conference 2018 Abstracts

Department/School

Institute for Marine and Antarctic Studies

Event title

Australian Society for Fish Biology Conference 2018

Event Venue

Melbourne

Date of Event (Start Date)

2018-10-07

Date of Event (End Date)

2018-10-11

Repository Status

  • Restricted

Socio-economic Objectives

Control of pests, diseases and exotic species in coastal and estuarine environments; Other environmental management not elsewhere classified

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