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Transient isotachophoresis-capillary zone electrophoresis for the analysis of paralytic shellfish toxins in mussel sample

conference contribution
posted on 2023-05-24, 14:40 authored by Abdul Keyon, AS, Rosanne Guijt, Christopher BolchChristopher Bolch, Michael BreadmoreMichael Breadmore
Accumulation of paralytic shellfish toxins (PSTs) in contaminated shellfish is a serious health risk making early detection important to improve shellfish safety and biotoxin management. Capillary electrophoresis (CE) has been proven as a high resolution separation technique compatible with miniaturization, making it an attractive choice in the development of portable instrumentation for early, on-site detection of PSTs. In this work, capillary zone electrophoresis (CZE) with capacitively coupled contactless conductivity detector (C4D) was compared with UV detection because of its small footprint. To improve sensitivity and deal with the high conductivity sample matrix, counter-flow transient isotachophoresis (tiTP) was used, exploiting the high sodium levels in the matrix as leading ion while L-alanine was used as the background electrolyte (BGE) and terminating electrolyte (TE). Careful optimization of the injected sample volume and duration of the counter-flow resulted in limit of detections (LODs) ranging from 74.2- 1020 ng/ml for tiTP-CZE-C4D and 141-461 ng/ml for tiTP-CZE-UV, an 8-97 fold over reduction compared to conventional CZE. The LODs were adequate for the analysis of PSTs in shellfish samples close to the regulatory limit (800 ng/ml). The developed method was applied to the analysis of a contaminated mussel sample and validated against results from Association of Official Analytical Chemists (AOAC)-approved method for PSTs analysis using pre-column oxidation of the sample prior to analysis by high performance liquid chromatography (HPLC) with fluorescence detection (FLO). The method presented has potential for incorporation into field-deployable devices for the early detection of PSTs on-site.


Publication title

The 16th International Conference on Harmful Algae Book of Abstracts




School of Natural Sciences

Event title

The 16th International Conference on Harmful Algae

Event Venue

Wellington, New Zealand

Date of Event (Start Date)


Date of Event (End Date)


Repository Status

  • Restricted

Socio-economic Objectives

Fisheries - aquaculture not elsewhere classified

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