17-β-Estradiol-mediated activation of extracellular-signal regulated kinase, phosphatidylinositol 3-kinase/protein kinase B-Akt and N-methyl-α-aspartate receptor phosphorylation in cortical synaptoneurosomes
journal contributionposted on 2023-05-19, 13:12 authored by Dominguez, R, Liu, R, Baudry, M
In addition to its well-known activational mechanism, the steroid hormone 17-β-estradiol (E2) has been shown to rapidly activate various signal transduction pathways that could participate in estrogen-mediated regulation of synaptic plasticity. Although the mechanisms underlying these effects are not clearly understood, it has been repeatedly suggested that they involve a plasma membrane receptor which has direct links to several intracellular signaling cascades. To further address the question of whether E2 acts directly at the synapse and through membrane-bound receptors, we studied the effects of E2 and of ligands of estrogen receptors on various signaling pathways in cortical synaptoneurosomes. Our results demonstrate that E2 elicits N-methyl-Daspartate receptor phosphorylation and activates the extracellular signal-regulated kinase and the phosphatidylinositol 3-kinase/Akt signal transduction pathways in this cortical membrane preparation. Furthermore, we provide evidence for the presence of a membrane-bound estrogen receptor responsible for these effects in cortical synaptoneurosomes. Our study demonstrates that E2 directly acts at cortical synapses, and that synaptoneurosomes provide a useful system to investigate the mechanisms by which E2 regulates synaptic transmission and plasticity.
Publication titleJournal of Neurochemistry
Department/SchoolTasmanian School of Medicine
PublisherBlackwell Publishing Ltd
Place of publicationOxford
Rights statementJournal Compilation © 2007 International Society for Neurochemistry. This is the peer reviewed version of the following article: Dominguez, R., Liu, R., Baudry, M., 2007. 17-β-Estradiol-mediated activation of extracellular-signal regulated kinase, phosphatidylinositol 3-kinase/protein kinase B-Akt and N-methyl-α-aspartate receptor phosphorylation in cortical synaptoneurosomes, Journal of neurochemistry, 10(1), 232-240, which has been published in final form at http://dx.doi.org/10.1111/j.1471-4159.2006.04360.x This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving.