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A More Cost- and Labour-Efficient Assay for the Combined Measurement of the Diastatic Power Enzymes β-Amylase, α-Amylase, and Limit Dextrinase¹
journal contribution
posted on 2023-05-16, 20:50 authored by Evans, EAn improved, combined extraction protocol in an 8 x 12 microwell format has been devised that satisfactorily estimates the levels of β-amylase, α-amylase, and limit dextrinase in malt. The improved protocol enables one person to undertake the assessment of 46 samples and 1 control in duplicate for each of the Ceralpha, Betamyl, and Limit DextriZyme assays, which require 5.5 hr of labor over 2 days, including an overnight extraction. The scaling down of the method resulted in the use of between one-quarter and one-fifth of the amount of substrate per assay recommended by the manufacturer, providing substantial cost savings. The increased labor and cost efficiencies described in this paper will increase the potential for diastatic power enzyme analyses to be undertaken as routine assays by malting quality evaluation laboratories, including breeding programs, maltsters, and grain traders.
Funding
Grains Research & Development Corporation
Archer Daniels Midland Company
Australian Grains Centre
Barrett Burston Maltings
Fosters Group Ltd
Joe White Maltings
Lion
History
Publication title
American Society of Brewing Chemists. JournalVolume
66Issue
4Pagination
215-222ISSN
0361-0470Department/School
Tasmanian Institute of Agriculture (TIA)Publisher
American Society of Brewing ChemistsPlace of publication
United StatesRepository Status
- Restricted