University Of Tasmania
154357 - a human adenovirus.pdf (7.67 MB)
Download file

A human adenovirus encoding IFN-γ can transduce Tasmanian devil facial tumour cells and upregulate MHC-I

Download (7.67 MB)
journal contribution
posted on 2023-05-21, 15:04 authored by Kayigwe, AN, Jocelyn DarbyJocelyn Darby, Alan LyonsAlan Lyons, Amanda PatchettAmanda Patchett, Lisowski, L, Guei-Sheung LiuGuei-Sheung Liu, Andrew FliesAndrew Flies
The devil facial tumour disease (DFTD) has led to a massive decline in the wild Tasmanian devil (Sarcophilus harrisii) population. The disease is caused by two independent devil facial tumours (DFT1 and DFT2). These transmissible cancers have a mortality rate of nearly 100 %. An adenoviral vector-based vaccine has been proposed as a conservation strategy for the Tasmanian devil. This study aimed to determine if a human adenovirus serotype 5 could express functional transgenes in devil cells. As DFT1 cells do not constitutively express major histocompatibility complex class I (MHC-I), we developed a replication-deficient adenoviral vector that encodes devil interferon gamma (IFN-γ) fused to a fluorescent protein reporter. Our results show that adenoviral-expressed IFN-γ was able to stimulate upregulation of beta-2 microglobulin, a component of MHC-I, on DFT1, DFT2 and devil fibroblast cell lines. This work suggests that human adenoviruses can serve as a vaccine platform for devils and potentially other marsupials.


Australian Research Council


Publication title

Journal of General Virology










Menzies Institute for Medical Research


Soc General Microbiology

Place of publication

Marlborough House, Basingstoke Rd, Spencers Woods, Reading, England, Berks, Rg7 1Ag

Rights statement

© 2022. The Authors. This article was made open-access via a Publish and Read agreement between the Microbiology Society and the corresponding author’s institution. This article is licensed under a Creative Commons Attribution 4.0 International (CC BY 4.0) License (, which permits use, sharing, adaptation, distribution, and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

Repository Status

  • Open

Socio-economic Objectives

Veterinary biological preventatives; Veterinary diagnostics; Expanding knowledge in the agricultural, food and veterinary sciences