Rao_et_al_2008_outX_JFR.pdf (289.17 kB)
A microsatellite study on outcrossing rates and contamination in an Eucalyptus globulus breeding arboretum
journal contribution
posted on 2023-05-16, 22:35 authored by RAO, H, Patterson, B, Bradley PottsBradley Potts, Rene VaillancourtRene VaillancourtFour pairs of microsatellite molecular polymorphism primers were used to analyse microsatellite fingerprints of 188 seedlings derived from an open-pollinated progeny grafted Eucalyptus globulus breeding arboretum in Victoria, south-eastern Australia. The microsatellite loci chosen for this study were highly polymorphic with the mean number of alleles per locus of 14.25. Individual mothers varied in their outcrosssing rate estimate from 15% to 95%, the overall outcrossing level in the arboretum was 47.9% and the contamination rate was 17.6%. The high selfing level was likely to result in marked inbreeding depression in the performance of open-pollinated seed lots. Open-pollinated seeds collected from such arboreta are not advisable because of its low genetic quality, although such arboreta may be useful for the seed production through large-scale manual pollination or collecting seeds only from trees or genotypes within the arboretum that have high outcrossing rates.
History
Publication title
Journal of Forestry ResearchVolume
19Pagination
136-140ISSN
1007-662XDepartment/School
School of Natural SciencesPublisher
Dongbei Linye DaxuePlace of publication
ChinaRepository Status
- Restricted