University Of Tasmania
2016 Ma Al QTL BMC Genimics.pdf (1.85 MB)

A new allele for aluminium tolerance gene in barley (Hordeum vulgare L.)

Download (1.85 MB)
journal contribution
posted on 2023-05-18, 17:36 authored by Ma, Y, Li, C, Ryan, PR, Sergey ShabalaSergey Shabala, You, J, Liu, J, Liu, C, Meixue ZhouMeixue Zhou

Background: Aluminium (Al) toxicity is the main factor limiting the crop production in acid soils and barley (Hordeum vulgare L.) is one of the most Al-sensitive of the small-grained cereals. The major gene for Al tolerance in barley is HvAACT1 (HvMATE) on chromosome 4H which encodes a multidrug and toxic compound extrusion (MATE) protein. The HvAACT1 protein facilitates the Al-activated release of citrate from root apices which protects the growing cells and enables root elongation to continue. A 1 kb transposable element-like insert in the 5’ untranslated region (UTR) of HvAACT1 is associated with increased gene expression and tolerance and a PCR-based marker is available to score for this insertion.

Results: We screened a wide range of barley genotypes for Al tolerance and identified a moderately tolerant Chinese genotype named CXHKSL which did not show the typical allele in the 5’ UTR of HvAACT1 associated with tolerance. We investigated the mechanism of Al tolerance in CXHKSL and concluded it also relies on the Al-activated release of citrate from roots. Quantitative trait loci (QTL) analysis of double haploid lines generated with CXHKSL and the Al-sensitive variety Gairdner mapped the tolerance locus to the same region as HvAACT1 on chromosome 4H.

Conclusions: Our results show that the Chinese barley genotype CXHKSL possesses a novel allele of the major Al tolerance gene HvAACT1.


Grains Research & Development Corporation


Publication title

BMC Genomics



Article number









Tasmanian Institute of Agriculture (TIA)


Biomed Central Ltd.

Place of publication

United Kingdom

Rights statement

© 2016 Ma et al. Licensed under Creative Commons Attribution 4.0 International (CC BY 4)

Repository Status

  • Open

Socio-economic Objectives


Usage metrics

    University Of Tasmania