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A new and selective capillary column coated with cationic diazacrown ether for separation of organic and inorganic anions by capillary electrophoresis
journal contribution
posted on 2023-05-16, 14:21 authored by Mori, M, Tsue, H, Tanaka, S, Tanaka, K, Paul HaddadPaul HaddadA new coated capillary has been introduced for capillary electrophoretic separation of anions by using a positively charged diazacrown ether with a 12-membered ring. A positive charge spread over the inner capillary surface led to a substantial anodic electroosmotic flow (EOF) over the range of migrating buffer of pH 2-11. Under the optimum conditions of 25 mM phosphate buffer at pH 7, the diazacrown-coated capillary showed a successful simultaneous separation of 7 inorganic anions and 13 aromatic anions (including positional isomers) in less than 15 min. The migration times of the sample anions and EOF marker for consecutive runs on a single column were highly reproducible, giving a relative standard deviation of 1%. Theoretical treatment of the migration behavior clearly demonstrated that ion association between the diazacrown and analyte anions is strongly dependent on the nature of the functional groups of anions (e.g., sulfonate groups carboxyl groups) and the number of negative charges (e.g., trivalent anions divalent anions monovalent anions) on the analyte.
History
Publication title
ElectrophoresisVolume
24Issue
12-13Pagination
1944-1950ISSN
0173-0835Department/School
School of Natural SciencesPublisher
Wiley-V C H Verlag GMBHPlace of publication
Weinheim, GermanyRights statement
The definitive published version is available online at: http://interscience.wiley.comRepository Status
- Restricted