posted on 2023-05-17, 23:00authored byTorda, G, Schmidt-Roach, S, Peplow, LM, Lundgren, P, van Oppen, MJH
Pocillopora damicornis (Linnaeus, 1758; Scleractinia, Pocilloporidae) has recently been found to comprise at least five distinct genetic lineages in Eastern Australia, some of which likely represent cryptic species. Due to similar and plastic gross morphology of these lineages, field identification is often difficult. Here we present a quick, cost effective genetic assay as well as three novel microsatellite markers that distinguish the two most common lineages found on the Great Barrier Reef. The assay is based on PCR amplification of two regions within the mitochondrial putative control region, which show consistent and easily identifiable fragment size differences for the two genetic lineages after Alu1 restriction enzyme digestion of the amplicons.
History
Publication title
PLoS ONE
Volume
8
Article number
e58447
Number
e58447
Pagination
1-5
ISSN
1932-6203
Department/School
Institute for Marine and Antarctic Studies
Publisher
Public Library of Science
Place of publication
USA
Rights statement
Copyright 2013 Torda et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.