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Download fileA rapid genetic assay for the identification of the most common Pocillopora damicornis genetic lineages on the Great Barrier Reef
journal contribution
posted on 2023-05-17, 23:00 authored by Torda, G, Schmidt-Roach, S, Peplow, LM, Lundgren, P, van Oppen, MJHPocillopora damicornis (Linnaeus, 1758; Scleractinia, Pocilloporidae) has recently been found to comprise at least five distinct genetic lineages in Eastern Australia, some of which likely represent cryptic species. Due to similar and plastic gross morphology of these lineages, field identification is often difficult. Here we present a quick, cost effective genetic assay as well as three novel microsatellite markers that distinguish the two most common lineages found on the Great Barrier Reef. The assay is based on PCR amplification of two regions within the mitochondrial putative control region, which show consistent and easily identifiable fragment size differences for the two genetic lineages after Alu1 restriction enzyme digestion of the amplicons.
History
Publication title
PLoS ONEVolume
8Article number
e58447Number
e58447Pagination
1-5ISSN
1932-6203Department/School
Institute for Marine and Antarctic StudiesPublisher
Public Library of SciencePlace of publication
USARights statement
Copyright 2013 Torda et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.Repository Status
- Open