posted on 2023-05-21, 12:59authored byMachado, JH, Ting, R, John LinJohn Lin, Rodriguez, EA
Self-labeling proteins have revolutionized super-resolution and sensor imaging. Tags recognize a bioorthogonal substrate for covalent attachment. We show the small Ultra-Red Fluorescent Protein (smURFP) is a self-labeling protein. The substrate is fluorogenic, fluoresces when attached, and quenches fluorescent cargo. The smURFP-tag has novel properties for tool development.
History
Publication title
RSC Chemical Biology
Issue
4
Pagination
1221-1226
ISSN
2633-0679
Department/School
Tasmanian School of Medicine
Publisher
Royal Society of Chemistry
Place of publication
Cambridge
Rights statement
Copyright 2021 The Authors Licensed under Creative Commons Attribution-NonCommercial 3.0 Unported (CC BY-NC 3.0) https://creativecommons.org/licenses/by-nc/3.0/