Acclimatization of skeletal muscle mitochondria to high-altitude hypoxia during an ascent of Everest
journal contribution
posted on 2023-05-18, 02:59authored byLevett, DZ, Radford, EJ, Menassa, DA, Graber, EF, Andrea Morash, Hoppeler, H, Clarke, K, Martin, DS, Ferguson-Smith, AC, Montgomery, HE, Grocott, MPW, Murray, AJ
Ascent to high altitude is associated with a fall in the partial pressure of inspired oxygen (hypobaric hypoxia). For oxidative tissues such as skeletal muscle, resultant cellular hypoxia necessitates acclimatization to optimize energy metabolism and restrict oxidative stress, with changes in gene and protein expression that alter mitochondrial function. It is known that lowlanders returning from high altitude have decreased muscle mitochondrial densities, yet the underlying transcriptional mechanisms and time course are poorly understood. To explore these, we measured gene and protein expression plus ultrastructure in muscle biopsies of lowlanders at sea level and following exposure to hypobaric hypoxia. Subacute exposure (19 d after initiating ascent to Everest base camp, 5300 m) was not associated with mitochondrial loss. After 66 d at altitude and ascent beyond 6400 m, mitochondrial densities fell by 21%, with loss of 73% of subsarcolemmalmitochondria. Correspondingly, levels of the transcriptional coactivator PGC-1α fell by 35%, suggesting down-regulation of mitochondrial biogenesis. Sustained hypoxia also decreased expression of electron transport chain complexes I and IV and UCP3 levels. We suggest that during subacute hypoxia, mitochondria might be protected from oxidative stress. However, following sustained exposure, mitochondrial biogenesis is deactivated and uncoupling down-regulated, perhaps to improve the efficiency of ATP production.
History
Publication title
The FASEB Journal
Volume
26
Issue
4
Pagination
1431-1441
ISSN
0892-6638
Publisher
Federation Amer Soc Exp Biol
Place of publication
9650 Rockville Pike, Bethesda, USA, Md, 20814-3998