University of Tasmania
Browse

File(s) under permanent embargo

Accurate phenotyping reveals better QTL for waterlogging tolerance in barley

journal contribution
posted on 2023-05-17, 02:51 authored by Meixue ZhouMeixue Zhou
Tolerance to soil waterlogging stress is an important plant breeding objective in high rainfall or poorly drained areas across many countries in the world. Similar to other abiotic stresses, waterlogging tolerance is a complicated trait and the selection for tolerance has been a major obstacle in breeding programmes. Molecular marker-assisted selection (MAS) can be very effective. However, the success of MAS depends on the development of reliable markers which requires accurate phenotyping when searching for the markers linked to the tolerance. In this experiment, a new screening facility, with the environment closer to field conditions, has been used to screen a double haploid population produced from a cross between waterlogging tolerant and susceptible varieties. A new scoring system of plant healthiness (0 = plant died from waterlogging; 10 = not affected by waterlogging) was also used in this experiment. The results were compared with a previous glasshouse pot experiment. Some quantitative trait loci (QTL) identified from the current experiment and previous pot experiment were quite different. From this experiment, two major QTL (QWL.YeFr.4H and QWL.YeFr.2H.2, accounting 24% and 17% of phenotypic variation) and two minor QTL (QWL.YeFr.2H.1 and QWL.YeFr.3H, accounting 8% and 7% of the variation) were found. These four markers can be very effectively used to select waterlogging tolerance. By selecting all four closely linked markers, all the lines will be quite tolerant with an average score of 7.6. In contrast, if none of above markers were selected all the lines would be susceptible with an average score of 1.4.

History

Publication title

Plant Breeding

Volume

130

Pagination

203-208

ISSN

0179-9541

Department/School

Tasmanian Institute of Agriculture (TIA)

Publisher

Blackwell Verlag Gmbh

Place of publication

Kurfurstendamm 57, Berlin, Germany, D-10707

Rights statement

The definitive published version is available online at: http://onlinelibrary.wiley.com/

Repository Status

  • Restricted

Socio-economic Objectives

Barley

Usage metrics

    University Of Tasmania

    Exports

    RefWorks
    BibTeX
    Ref. manager
    Endnote
    DataCite
    NLM
    DC