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Acquired macrolide resistance genes in Haemophilus influenzae?

journal contribution
posted on 2023-05-18, 10:40 authored by Atkinson, CT, Kunde, DA, Stephen TristramStephen Tristram

Objectives: The objective of this study was to determine the prevalence of specific acquired macrolide resistance genes previously reported as present in clinical isolates of Haemophilus influenzae.

Methods: A collection of 172 clinical respiratory isolates of H. influenzae, including 59 isolates from cystic fibrosis patients and 27 from non-cystic fibrosis bronchiectasis patients with significant prior macrolide use, was established. This collection was tested for azithromycin susceptibility using Etest and screened for the presence of erm(A), erm(B), erm(C), erm(F), mef(A) and mef(E) using locked nucleic acid dual-labelled hydrolysis probes.

Results: The azithromycin MICs ranged from 0.09 to > 256 mg/L, with 2 (1.2%) isolates susceptible, 163 (94.8%) intermediate and 7 (4%) resistant according to EUCAST breakpoints (susceptible, ≤ 0.12 mg/L; resistant, >4 mg/L). None of the acquired macrolide resistance genes erm(A), erm(B), erm(C), erm(F), mef(A) or mef(E) was detected in any of the isolates.

Conclusions: The specific acquired macrolide resistance genes are not widespread in H. influenzae and the high prevalence of these genes previously reported might be unique to the specific circumstances of that study.

Funding

Clifford Craig Foundation

History

Publication title

Journal of Antimicrobial Chemotherapy

Volume

70

Issue

8

Pagination

2234-2236

ISSN

0305-7453

Department/School

School of Health Sciences

Publisher

Oxford Univ Press

Place of publication

United Kingdom

Rights statement

Copyright 2015 The Author

Repository Status

  • Restricted

Socio-economic Objectives

Clinical health not elsewhere classified

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    University Of Tasmania

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