File(s) not publicly available
An Fgfr3-iCreER(T2) Transgenic Mouse Line for Studies of Neural Stem Cells and Astrocytes
journal contribution
posted on 2023-05-17, 08:36 authored by Kaylene YoungKaylene Young, Mitsumori, T, Pringle, N, Grist, M, Kessaris, N, Richardson, WDThe lack of markers for astrocytes, particularly gray matter astrocytes, significantly hinders research into their development and physiological properties. We previously reported that fibroblast growth factor receptor 3 (Fgfr3) is expressed by radial precursors in the ventricular zone of the embryonic neural tube and subsequently by differentiated astrocytes in gray and white matter. Here, we describe an Fgfr3-iCreERT2 phage artificial chromosome transgenic mouse line that allows efficient tamoxifen-induced Cre recombination in Fgfr3-expressing cells, including radial glial cells in the embryonic neural tube and both fibrous and protoplasmic astrocytes in the mature central nervous system. This mouse strain will therefore be useful for studies of normal astrocyte biology and their responses to CNS injury or disease. In addition, Fgfr3-iCreERT2 drives Cre recombination in all neurosphere-forming stem cells in the adult spinal cord and at least 90% of those in the adult forebrain subventricular zone. We made use of this to show that there is continuous accumulation of all major interneuron subtypes in the olfactory bulb (OB) from postnatal day 50 (P50) until at least P230 (∼8 months of age). It therefore seems likely that adult-born interneurons integrate into existing circuitry and perform long-term functions in the adult OB. 2010 Wiley-Liss, Inc.
History
Publication title
GliaVolume
58Issue
8Pagination
943-953ISSN
0894-1491Department/School
Menzies Institute for Medical ResearchPublisher
Wiley-LissPlace of publication
Div John Wiley & Sons Inc, 605 Third Ave, New York, USA, Ny, 10158-0012Repository Status
- Restricted