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Analysis of flavonoids by non-aqueous capillary electrophoresis with 1-ethyl-3-methylimidazolium ionic-liquids as background electrolytes

journal contribution
posted on 2023-05-17, 21:36 authored by Lu, Y, Jia, C, Yao, Q, Zhong, H, Michael BreadmoreMichael Breadmore
The separation of three flavonoids, kaempferol, quercetin and luteolin, by capillary zone electrophore-sis with three 1-ethyl-3-methylimidazolium ionic liquids (ILs), namely chloride, hydrogen sulfate andtetrafluoroborate salts, under non-aqueous conditions using acetonitrile as solvent was investigated.Depending on the IL, the three flavonoids can be separated under positive voltage with a low IL concentra-tion and negative voltage with a high IL concentration. The separations are based on heteroconjungationbetween the IL anions and the analytes. The heteroconjungation between the anions of the ILs and theanalytes is proton sensitive and only a very small amount of protic solvents, such as methanol, addedinto the electrolyte solution can harm the separation, but higher IL concentrations can overcome thishigher amount of protic solvents. Using a high concentration of IL in the sample was used to enhancethe flavonoid solubility by as much as 10, overcoming the problem of the low solubility during NACEanalysis with ACN as the solvent. Finally, kaempferol, quercetin and luteolin in Semen Plantaginis wereanalysed by nonaqueous capillary electrophoresis with low concentration of the hydrogen sulfate ionicliquid with excellent results.

Funding

Australian Research Council

History

Publication title

Journal of Chromatography A

Volume

1319

Pagination

160-165

ISSN

0021-9673

Department/School

School of Natural Sciences

Publisher

Elsevier BV

Place of publication

Netherlands

Rights statement

Copyright 2013 Elsevier B.V.

Repository Status

  • Restricted

Socio-economic Objectives

Expanding knowledge in the chemical sciences

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