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Analysis of microbial hydrocarbon degradation using TLC-FID

journal contribution
posted on 2023-05-16, 07:40 authored by Cavanagh, JE, Juhasz, AL, Peter Nichols, Franzmann, PD, Thomas McMeekinThomas McMeekin
Biodegradation of the hydrocarbons, tetradecane and phenanthrene, by mixed and pure cultures of Antarctic bacteria was measured using thin layer chromatography-flame ionisation detection (TLC-FID). Losses of up to 65% of tetradecane and complete degradation of phenanthrene with concurrent increases in bacterial biomass, measured by polar lipid concentration, were observed. TLC-FID has advantages over other analytical techniques used to monitor hydrocarbon biodegradation by enabling a number of samples to be replicated and run simultaneously in a short period of time. TLC-FID analysis enables three samples to be run in triplicate and analysed in 45 min. Analysis time may be further reduced if only one compound class is present in the samples. The TLC-FID system can detect concentrations as low as 0.1 μg/μl, as applied on the chromarods, with a reproducibility of ± 10%. Biodegradation of low molecular weight hydrocarbons, while not accurately quantitated using TLC-FID, could be qualitatively assessed using this system.

History

Publication title

Journal of Microbiological Methods

Volume

22

Pagination

119-130

ISSN

0167-7012

Department/School

Tasmanian Institute of Agriculture (TIA)

Publisher

Elsevier Science Bv

Place of publication

USA

Repository Status

  • Restricted

Socio-economic Objectives

Other environmental management not elsewhere classified

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