University Of Tasmania

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Analytical isotachophoresis of lactate in human serum using dry film photoresist microfluidic chips compatible with a commercially available field-deployable instrument platform

journal contribution
posted on 2023-05-17, 21:37 authored by Petr SmejkalPetr Smejkal, Michael BreadmoreMichael Breadmore, Rosanne Guijt, Foret, F, Bek, F, Miroslav MackaMiroslav Macka
A dry film resist (DFR) chip compatible with the Agilent Bioanalyzer 2100 was designed and fabricated for use in the analysis of lactate in serum by chip isotachophoresis (ITP). The Agilent Bioanalyzer 2100 is a commercially available field deployable analytical instrument originally developed for the elec-trophoretic analysis of DNA, RNA and proteins. The DFR chip was designed for the ITP separation of lactate in human serum within 1 min and was made compatible with the Bioanalyzer after packaging in the plastic caddies normally used for the DNA chips. A 20-fold improvement in sensitivity was obtained for the DFR chips in comparison with the standard chips used in earlier work. The limit of detection and limit of quantification for lactate were 24 uM and 80 uM, respectively. This new approach enables the use of commercial platforms like the Agilent Bioanalyzer for new applications including the analysis of small molecules.


Australian Research Council


Publication title

Analytica Chimica Acta








School of Natural Sciences


Elsevier BV

Place of publication


Rights statement

Copyright 2013 Elsevier

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  • Restricted

Socio-economic Objectives

Expanding knowledge in the chemical sciences