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Anionic microemulsion to solvent stacking for on-line sample concentration of cationic analytes in capillary electrophoresis

journal contribution
posted on 2023-05-18, 07:28 authored by Kukusamude, C, Srijaranai, S, Joselito Quirino
The common SDS microemulsion (i.e. 3.3% SDS, 0.8% octane, and 6.6% butanol) and organic solvents were investigated for the stacking of cationic drugs in capillary zone electrophoresis using a low pH separation electrolyte. The sample was prepared in the acidic microemulsion and a high percentage of organic solvent was included in the electrolyte at anodic end of capillary. The stacking mechanism was similar to micelle to solvent stacking where the micelles were replaced by the microemulsion for the transport of analytes to the organic solvent rich boundary. This boundary is found between the microemulsion and anodic electrolyte. The effective electrophoretic mobility of the cations reversed from the direction of the anode in the microemulsion to the cathode in the boundary. Microemulsion to solvent stacking was successfully achieved with 40% ACN in the anodic electrolyte and hydrodynamic sample injection of 21 s at 1000 mbar (equivalent to 30% of the effective length). The sensitivity enhancement factors in terms of peak height and corrected peak area were 15 to 35 and 21 to 47, respectively. The linearity R2 in terms of corrected peak area were > 0.999. Interday precisions (%RSD, n = 6) were 3.3–4.0% for corrected peak area and 2.0–3.0% for migration time. Application to spiked real sample is also presented.

History

Publication title

Electrophoresis

Volume

35

Issue

10

Pagination

1478-1483

ISSN

0173-0835

Department/School

School of Natural Sciences

Publisher

Wiley-V C H Verlag Gmbh

Place of publication

PO Box 10 11 61, Weinheim, Germany, D-69451

Rights statement

Copyright 2014 Wiley-VCH Verlag

Repository Status

  • Restricted

Socio-economic Objectives

Expanding knowledge in the chemical sciences

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