Plantation-grown Eucalyptus nitens (E. nitens) has been grown predominantly for the pulp and paper industry. In this study, the suitability of E. nitens as a structural material is examined using static tensile tests in a universal testing machine. The anisotropic tensile behaviour of 240 Eucalyptus nitens small clear wood samples with a diversity of grain angles was examined in both dry and wet conditions. The samples had a highly anisotropic tensile characterisation in the context of both a low moisture content (MC = 12%) and a high moisture content (MC > its fibre saturation point, FSP). The results showed that, in a high moisture content condition, the wood showed a lower failure strength and more ductility at all grain angles than in a low moisture content condition. The underlying failure mechanism of Eucalyptusnitens timber in tension was determined in detail from the perspective of the microstructure of wood cellulose polymer composites. The mean tensile failure strengths perpendicular and parallel to the fibre direction were, respectively, 5.6 and 91.6 MPa for the low MC and 3.8 and 62.1 MPa for the high MC condition. This research provides a basis for using E. nitens as a potential structural tensile member. The moisture modification factors of Eucalyptus timber at a mean level are higher than those of the traditional construction material, Pinus radiata, implying that E. nitens is promising as a material to be used for tensile members in water saturated conditions.