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Download fileAnti-PML-RARα shRNA sensitises promyelocytic leukaemia cells to all-trans retinoic acid
The PML-RARα fusion gene disrupts the retinoic acid differentiation signal in a range of leukaemia types, promoting proliferation. We designed a shRNA to target the fusion mRNA, and the shRNA expression cassette was delivered via lentiviral transduction. Delivery of this shRNA significantly down regulated the target mRNA, with effects also evident at the protein level. When combined with ATRA administration, this down regulation of the fusion gene strongly inhibited proliferation in the NB4 PML cell line.
History
Publication title
Journal of RNAi and Gene SilencingVolume
8Pagination
464-469ISSN
1747-0854Department/School
Menzies Institute for Medical ResearchPublisher
Library Publishing MediaPlace of publication
United StatesRights statement
Licenced under Creative Commons Attribution-NonCommercial 2.5 Generic (CC BY-NC 2.5) http://creativecommons.org/licenses/by-nc/2.5/Repository Status
- Open