Anti-PML-RARα shRNA sensitises promyelocytic leukaemia cells to all-trans retinoic acid
journal contributionposted on 2023-05-17, 15:29 authored by Casey, NP, Gregory WoodsGregory Woods
The PML-RARα fusion gene disrupts the retinoic acid differentiation signal in a range of leukaemia types, promoting proliferation. We designed a shRNA to target the fusion mRNA, and the shRNA expression cassette was delivered via lentiviral transduction. Delivery of this shRNA significantly down regulated the target mRNA, with effects also evident at the protein level. When combined with ATRA administration, this down regulation of the fusion gene strongly inhibited proliferation in the NB4 PML cell line.
Publication titleJournal of RNAi and Gene Silencing
Department/SchoolMenzies Institute for Medical Research
PublisherLibrary Publishing Media
Place of publicationUnited States
Rights statementLicenced under Creative Commons Attribution-NonCommercial 2.5 Generic (CC BY-NC 2.5) http://creativecommons.org/licenses/by-nc/2.5/