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PES 70183 2010 Silva Britz actascibiolsci.pdf (491.64 kB)

Application of molecular fingerprinting for analysis of a PAH-contaminated soil microbiota growing in the presence of complex PAHs

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posted on 2023-05-17, 06:20 authored by Silva, IS, De Menezes, CR, De Faria, AF, Da Costa, JG, Pepper, S, Margaret BritzMargaret Britz, Durrant, LR
Polycyclic aromatic hydrocarbons (PAHs) constitute a group of priority pollutants which are present at high concentrations in the soils of many industrial contaminated sites. Pollution by these compounds may stimulate growth of organisms able to live in these environments causing changes in the structure of the microbial community due to some cooperative process of metabolization of toxic compounds. A long-term PAH-contaminated soil was stored for several years and used to analyze the native microbiota regarding their ability to grow on pyrene, benzo[a]pyrene, as well as in mixtures of LMW- and HMW-PAHs. Molecular profiles of the microbial community was assessed by PCR-DGGE of 16S rRNA gene, and the number of bands observed in DGGE analyses was interpreted as dominant microbial members into the bacterial community. Results of PAH-contaminated soil microorganisms showed different profiles in the degradative dynamics when some nutrients were added. Predominant species may play a significative role while growing and surviving on PAHs, and some other metabolically active species have emerged to interact themselves in a cooperative catabolism of PAHs.

History

Publication title

Acta Scientiarum. Biological Sciences

Volume

32

Pagination

63-69

ISSN

1679-9283

Department/School

Tasmanian Institute of Agriculture (TIA)

Publisher

Universidade Estadual de Maringa * Editora da Universidade - Eduem

Place of publication

Brazil

Rights statement

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. http://creativecommons.org/licenses/by/2.0/

Repository Status

  • Open

Socio-economic Objectives

Evaluation, allocation, and impacts of land use

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