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Approaches for the sensitive detection of rare base and prime editing events
journal contributionposted on 2023-05-21, 00:14 authored by Minh Thuan Nguyen Tran, Rajendra KCRajendra KC, Freya PattersonFreya Patterson, Guei-Sheung LiuGuei-Sheung Liu, Anthony CookAnthony Cook, Alexander HewittAlexander Hewitt
Precision chemistry entailing user-directed nucleotide substitutions and template-specified repair can be facilitated by base editing and prime editing, respectively. Recently, the diversification of adenine, cytosine, and prime editor variants obliges a considered, high-throughput evaluation of these tools for optimized, end-point applications. Herein, we outline novel, cost-effective and scalable approaches for the rapid detection of base editing and prime editing outcomes using gel electrophoresis. For base editing, we exploit primer mismatch amplification (SNP genotyping) for the gel-based detection of base editing efficiencies as low as 0.1%. For prime editing, we describe a one-pot reaction combining polymerase chain reaction (PCR) amplification of the target region with restriction digestion (restriction fragment length polymorphism; RFLP). RFLP enables the rapid detection of insertion or deletion events in under 2.5 h from genomic DNA extraction. We show that our method of SNP genotyping is amenable to both endogenous target loci as well as transfected, episomal plasmid targets in BHK-21 cells. Next, we validate the incidence of base and prime editing by describing Sanger sequencing and next-generation sequencing (NGS) workflows for the accurate validation and quantification of on-target editing efficiencies. Our workflow details three different methods for the detection of rare base and prime editing events, enabling a tiered approach from low to high resolution that makes use of gel electrophoresis, Sanger sequencing, and NGS.
Department/SchoolMenzies Institute for Medical Research
PublisherAcademic Press Inc Elsevier Science
Place of publication525 B St, Ste 1900, San Diego, USA, Ca, 92101-4495
Rights statement© 2021 Elsevier Inc. All rights reserved.