Abstract & Context Stem straightness is an important trait for growers and processors of Eucalyptus globulus logs for solid-wood products. & Aims The aims of the study were to determine the extent of genetic variation in stem deviation from straightness in E. globulus and assess the utility of a six-point subjective scoring method as a selection criterion for stem straightness. & Methods Two E. globulus progeny trials, grown under solid-wood product regimes, were studied. At age 9 years (post-thinning), stem straightness was measured using both image analysis and a six-point subjective scale. Diameter at breast height (DBH; 1.3 m) was measured at both age 5 (pre-thinning) and age 9 years. & Results Significant additive genetic variation was observed. Strong, positive and significant additive genetic correlations were observed between the stem straightness assessment methods and between DBH at ages 5 and 9 years. Significant positive genetic correlations were shown between subjectively scored stem straightness and DBH at both ages 5 and 9 years. & Conclusion The six-point subjective scoring method is a cost-effective selection criterion for stem straightness in E. globulus. The image measurement technique may be applied where objective estimates of stem straightness are required, for training purposes and to verify subjective scores. Keywords Eucalyptus globulus . Heritability . Correlation . Stem straightness . Image analysis