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Attached and planktonic Listeria monocytogenes global proteomic responses and associated influence of strain genetics and temperature

journal contribution
posted on 2023-05-18, 12:13 authored by Mata, MM, da Silva, WP, Richard WilsonRichard Wilson, Lowe, E, John BowmanJohn Bowman
Contamination of industrial and domestic food usage environments by the attachement of bacterial food-borne pathogen Listeria monocytogenes has public health and economic implications. Comprehensive proteomics experiments using label-free liquid chromatography/tandem mass spectrometry were used to compare the proteomes of two different L. monocytogenes strains (Siliken_1/2c and F2365_4b), which show very different capacities to attach to surfaces. Growth temperature and strain type were highly influential on the proteomes in both attached and planktonic cells. On the basis of the proteomic data, it is highly unlikely that specific surface proteins play a direct role in adherence to inanimate surfaces. Instead, strain-dependent responses related to cell envelope polymer biosynthesis and stress response regulation likely contribute to a different ability to attach and also to survive external stressors. Collectively, the divergent proteome-level responses observed define strain- and growth-temperature-dependent differences relevant to attachment efficacy, highlight relevant proteins involved in stress protection in attached cells, and suggest that strain differences and growth conditions are important in relation to environmental persistence.

History

Publication title

Journal of Proteome Research

Volume

14

Pagination

1161-1173

ISSN

1535-3893

Department/School

Tasmanian Institute of Agriculture (TIA)

Publisher

Amer Chemical Soc

Place of publication

1155 16Th St, Nw, Washington, USA, Dc, 20036

Rights statement

© 2014 American Chemical Society

Repository Status

  • Restricted

Socio-economic Objectives

Food safety

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