140298 - Bacteriomic profiling of branchial lesions induced by Neoparamoeba perurans.pdf (5.01 MB)
Download fileBacteriomic profiling of branchial lesions induced by Neoparamoeba perurans challenge reveals commensal dysbiosis and an association with Tenacibaculum dicentrarchi in AGD-affected Atlantic salmon (Salmo Salar L.)
journal contribution
posted on 2023-05-20, 16:43 authored by Slinger, J, Mark AdamsMark Adams, Wynne, JW Amoebic gill disease is a parasitic condition that commonly affects marine farmed Atlantic salmon. The causative agent, Neoparamoeba perurans, induces a marked proliferation of the gill mucosa and focal superficial necrosis upon branchial lesions. The effect that amoebic branchialitis has upon gill associated commensal bacteria is unknown. A 16S rRNA sequencing approach was employed to profile changes in bacterial community composition, within amoebic gill disease (AGD)-affected and non-affected gill tissue. The bacterial diversity of biopsies with and without diseased tissue was significantly lower in the AGD-affected fish compared to uninfected fish. Furthermore, within the AGD-affected tissue, lesions appeared to contain a significantly higher abundance of the Flavobacterium, Tenacibaculum dicentrarchi compared to adjunct unaffected tissues. Quantitative PCR specific to both N. perurans and T. dicentrarchi was used to further examine the co-abundance of these known fish pathogens. A moderate positive correlation between these pathogens was observed. Taken together, the present study sheds new light on the complex interaction between the host, parasite and bacterial communities during AGD progression. The role that T. dicentrarchi may play in this complex relationship requires further investigation.
History
Publication title
MicroorganismsVolume
8Issue
8Article number
1189Number
1189Pagination
1-17ISSN
2076-2607Department/School
Institute for Marine and Antarctic StudiesPublisher
MDPIAGPlace of publication
SwitzerlandRights statement
Copyright 2020 by the authors. Licensed under Creative Commons Attribution 4.0 International (CC BY 4.0) https://creativecommons.org/licenses/by/4.0/Repository Status
- Open