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Biochemical properties of a keratan sulphate/chondroitin sulphate proteoglycan expressed in primate pluripotent stem cells
journal contributionposted on 2023-05-17, 02:41 authored by Cooper, S, William BennettWilliam Bennett, Andrade, J, Reubinoff, BE, Thomson, J, Pera, MF
We previously identified a pericellular matrix keratan sulphate/chondroitin sulphate proteoglycan present on the surface of human embryonal carcinoma stem cells, cells whose differentiation mimics early development. Antibodies reactive with various epitopes on this molecule define a cluster of differentiation markers for primate pluripotent stem cells. We describe the purification of a form of this molecule which is secreted or shed into the culture medium. Biochemical analysis of the secreted form of this molecule shows that the monomeric form, whilst containing keratan sulphate, resembles mucins in its structure and its modification with O-linked carbohydrate. Immunofluorescence and immunoblotting data show that monkey and human pluripotent stem cells react with antibodies directed against epitopes on either carbohydrate side chains or the protein core of the molecule.
Publication titleJournal of Anatomy: Molecular, Cellular and Experimental Morphology
Department/SchoolMenzies Institute for Medical Research
PublisherBlackwell Publishing Ltd
Place of publication9600 Garsington Rd, Oxford, England, Oxon, Ox4 2Dg