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Biosynthesis of the halogenated auxin, 4-chloroindole-3-acetic acid
journal contribution
posted on 2023-05-17, 12:38 authored by Nathan TivendaleNathan Tivendale, Davidson, SE, Noel DaviesNoel Davies, Jason SmithJason Smith, Dalmais, M, Bendahmane, AI, Quittenden, LJ, Josh Sutton Smith, Bala, RK, Le Signor, C, Thompson, R, Henry HorneHenry Horne, James ReidJames Reid, John RossJohn RossSeeds of several agriculturally important legumes are rich sources of the only halogenated plant hormone, 4-chloroindole-3- acetic acid. However, the biosynthesis of this auxin is poorly understood. Here, we show that in pea (Pisum sativum) seeds, 4-chloroindole-3-acetic acid is synthesized via the novel intermediate 4-chloroindole-3-pyruvic acid, which is produced from 4-chlorotryptophan by two aminotransferases, TRYPTOPHAN AMINOTRANSFERASE RELATED1 and TRYPTOPHAN AMINOTRANSFERASE RELATED2. We characterize a tar2 mutant, obtained by Targeting Induced Local Lesions in Genomes, the seeds of which contain dramatically reduced 4-chloroindole-3-acetic acid levels as they mature. We also show that the widespread auxin, indole-3-acetic acid, is synthesized by a parallel pathway in pea.
History
Publication title
Plant PhysiologyVolume
159Pagination
1055-1063ISSN
0032-0889Department/School
School of Natural SciencesPublisher
Amer Soc Plant BiologistsPlace of publication
15501 Monona Drive, Rockville, USA, Md, 20855Rights statement
Copyright 2012 American Society of Plant BiologistsRepository Status
- Restricted