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Blockage of Brassinosteroid Biosynthesis and Sensitivity Causes Dwarfism in Garden Pea
journal contributionposted on 2023-05-16, 10:39 authored by Nomura, T, Nakayama, M, James ReidJames Reid, Takeuchi, Y, Yokota, T
Endogenous brassinosteroids (BRs) in the dwarf mutants lka and lkb of garden pea (Pisum sativum L.) and comparable wild-type plants were quantified by gas chromatography-selected ion monitoring using deuterated internal standards. In young shoots of the lkb mutant, the levels of brassinolide, castasterone, and 6-deoxocastasterone were 23-, 22-, and 9-fold lower, respectively, than those of wild-type plants. Applications of brassinolide, cas-tasterone, typhasterol, 3-dehydroteasterone, and teasterone normalized internode growth of lkb seedlings. These findings indicate that the lkb plants are BR-deficient mutants, probably as a consequence of a block in the BR biosynthetic pathway prior to the production of teasterone. Young shoots of lka plants contained only 50% less brassinolide and 5 times more castasterone than the equivalent wild-type tissues. The lka seedlings were approximately 100 times less responsive to brassinolide than the lkb mutant, and application of castasterone had only a marginal effect on lka internode growth, suggesting that the lka lesion results in impaired sensitivity to BR.
Publication titlePlant Physiol
Department/SchoolSchool of Natural Sciences
PublisherAmer Soc Plant Biologists
Place of publicationUSA