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CD8 alpha- and Langerin-negative dendritic cells, but not Langerhans cells, act as principal antigen-presenting cells in leishmaniasis
journal contributionposted on 2023-05-17, 07:46 authored by Ritter, U, Meissner, A, Scheidig, C, Heinrich KornerHeinrich Korner
In the early phase of leishmaniasis three types of potential antigen-presenting cells, including epidermal Langerhans cells (LC), dermal dendritic cells (DC) and inflammatory DC, are localized at the site of infection. Therefore, it has been a central question which cell type is responsible for the initiation of a protective immune response. In the early stage of an anti-Leishmania immune response, detectable Leishmania major antigen was localized in the paracortex of the draining lymph nodes (LN). Characterization of antigen-positive cells showed that L. major co-localized with DC of a CD11c+ CD8Î± - Langerin- phenotype. To determine the area of antigen uptake, dermis or epidermis, and to further define the type of antigen -transporting cells, L. major was inoculated subcutaneously and concurrently LC were mobilized with fluorescein isothiocyanate (FITC . After 3 days, DC carrying L. major antigen were always FITC-, indicating a dermal and not an epidermal origin. Moreover, addition of L. major antigen to ex vivo isolated CD8Î± - and CD8Î±+ DC from the draining LN of L. major-infected C57BL/6 mice demonstrated that both DC subpopulations were able to stimulate antigen-specific T cell proliferation in vitro. Without addition of exogenous antigen only the CD8Î±- Langerin- DC were capable of stimulating antigen-specific T cell proliferation. Thus, we demonstrate that CD8Î±- Langerin DC and not LC are the basis of the protective immune response to intracellular L. major parasites in vivo. Â© 2004 Wiley-VCH Verlag GmbH & Co. KGaA.
Publication titleEuropean Journal of Immunology
Department/SchoolMenzies Institute for Medical Research
PublisherWiley-V C H Verlag Gmbh
Place of publicationPo Box 10 11 61, Weinheim, Germany, D-69451