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CYP450s analysis across spiny lobster metamorphosis identifies a long sought missing link in crustacean development

journal contribution
posted on 2023-05-19, 06:29 authored by Ventura, T, Bose, U, Quinn FitzgibbonQuinn Fitzgibbon, Gregory SmithGregory Smith, Shaw, PN, Cummins, SF, Elizur, A
Cytochrome P450s (CYP450s) are a rapidly evolving family of enzymes, making it difficult to identify bona fide orthologs with notable lineage-specific exceptions. In ecdysozoans, a small number of the most conserved orthologs include enzymes which metabolize ecdysteroids. Ecdysone pathway components were recently shown in a decapod crustacean but with a notable absence of shade, which is important for converting ecdysone to its active form, 20-hydroxyecdysone (20HE), suggesting that another CYP450 performs a similar function in crustaceans. A CYPome temporal expression analysis throughout metamorphosis performed in this research highlights several un-annotated CYP450s displaying differential expression and provides information into expression patterns of annotated CYP450s. Using the expression patterns in the Eastern spiny lobster Sagmariasus verreauxi, followed by 3D modelling and finally activity assays in vitro, we were able to conclude that a group of CYP450s, conserved across decapod crustaceans, function as the insect shade. To emphasize the fact that these genes share the function with shade but are phylogenetically distinct, we name this enzyme system Shed.

Funding

Australian Research Council

History

Publication title

Journal of Steroid Biochemistry and Molecular Biology

Volume

171

Pagination

262-269

ISSN

0960-0760

Department/School

Institute for Marine and Antarctic Studies

Publisher

Pergamon-Elsevier Science Ltd

Place of publication

The Boulevard, Langford Lane, Kidlington, Oxford, England, Ox5 1Gb

Rights statement

Crown Copyright © 2017 Published by Elsevier

Repository Status

  • Restricted

Socio-economic Objectives

Fisheries - aquaculture not elsewhere classified

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    University Of Tasmania

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