Calibration of the HemoCue point-of-care analyser for determining hemoglobin concentration in a lizard and a fish

Hemoglobin concentration [Hb] is measured for a wide variety of animal studies. The use of Point-of-care devices, such as the HemoCue, is becoming increasingly common due to their portability, relative ease of use and low cost. This study aimed to determine whether the [Hb] of blue-tongued skink (Tiliqua nigrolutea) blood can be accurately determined using the HemoCue and whether the HemoCue overestimates [Hb] of reptile blood in a similar manner to fish blood. We additionally aimed to test whether ploidy affected [Hb] determined by the HemoCue using blood from diploid and triploid Atlantic salmon (Salmo salar). The HemoCue Hb 201⁺ systematically overestimated [Hb] in both blue-tongued skinks and Atlantic salmon and there was no difference between calibration equations determined for diploid or triploid salmon. The overestimation was systematic in both species, and as such, [Hb] determined by the HemoCue can be corrected using appropriate calibration equations.