155474 - can maternal exposure to air pollution.pdf (8.04 MB)
Can maternal exposure to air pollution affect post-natal liver development?
journal contributionposted on 2023-05-21, 16:34 authored by Yong SongYong Song, Chen, L, Ellen BennettEllen Bennett, Amanda WheelerAmanda Wheeler, Katherine SouthamKatherine Southam, Seiha YenSeiha Yen, Fay JohnstonFay Johnston, Graeme ZoskyGraeme Zosky
Emerging evidence suggests that inhalation of particulate matter (PM) can have direct adverse effects on liver function. Early life is a time of particular vulnerability to the effects of air pollution. On that basis, we tested whether in utero exposure to residential PM has an impact on the developing liver. Pregnant mice (C57BL/6J) were intranasally administered 100 µg of PM sampled from residential roof spaces (~5 mg/kg) on gestational days 13.5, 15.5, and 17.5. The pups were euthanized at two weeks of age, and liver tissue was collected to analyse hepatic metabolism (glycogen storage and lipid level), cellular responses (oxidative stress, inflammation, and fibrosis), and genotoxicity using a range of biochemical assays, histological staining, ELISA, and qPCR. We did not observe pronounced effects of environmentally sampled PM on the developing liver when examining hepatic metabolism and cellular response. However, we did find evidence of liver genomic DNA damage in response to in utero exposure to PM. This effect varied depending on the PM sample. These data suggest that in utero exposure to real-world PM during mid-late pregnancy has limited impacts on post-natal liver development.
Department/SchoolMenzies Institute for Medical Research
Place of publicationSwitzerland
Rights statementCopyright (2023) the authors. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CCBY) license (https:// creativecommons.org/licenses/by/ 4.0/).