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Capillary electrophoresis for automated on-line monitoring of suspension cultures: Correlating cell density, nutrients and metabolites in near real-time
journal contributionposted on 2023-05-18, 18:44 authored by Alhusban, AA, Michael BreadmoreMichael Breadmore, Nuri GuvenNuri Guven, Rosanne Guijt
Increasingly stringent demands on the production of biopharmaceuticals demand monitoring of process parameters that impact on their quality. We developed an automated platform for on-line, near real-time monitoring of suspension cultures by integrating microfluidic components for cell counting and filtration with a high-resolution separation technique. This enabled the correlation of the growth of a human lymphocyte cell line with changes in the essential metabolic markers, glucose, glutamine, leucine/isoleucine and lactate, determined by Sequential Injection-Capillary Electrophoresis (SI-CE). Using 8.1 mL of media (41 μL per run), the metabolic status and cell density were recorded every 30 min over 4 days. The presented platform is flexible, simple and automated and allows for fast, robust and sensitive analysis with low sample consumption and high sample throughput. It is compatible with up- and out-scaling, and as such provides a promising new solution to meet the future demands in process monitoring in the biopharmaceutical industry.
Publication titleAnalytica Chimica Acta
Department/SchoolSchool of Pharmacy and Pharmacology
PublisherElsevier Science Bv
Place of publicationNetherlands
Rights statementCopyright 2016 Elsevier B.V.