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Characterisation of a type IV pilus produced by Aeromonas caviae
journal contributionposted on 2023-05-16, 11:21 authored by Kirov, SM, Sanderson, K, Tracey DicksonTracey Dickson
A pilus produced by a clinical isolate of Aeromanas caviae (strain CA195) was purified and partially characterised. The M(r) of the pilin was estimated to be 23 kDa by SDS-PAGE. Its N-terminal amino-acid sequence showed that it was closely related to 'bundle-forming' type IV pili purified from other Aeromonas spp. associated with gastro-enteritis and considered to be important intestinal colonisation factors. Bundle-forming pili, often with a polar location, were seen on the surface of strain CA195 which was highly adherent to HEp-2 cells. Removal of surface structures by mechanical means decreased adhesion (by â‰¤ 50%) suggesting that these pill played some role in HEp-2 cell binding. This pilus type could prove an important marker for enteropathogenic A. caviae which appear to lack other putative virulence factors.
Publication titleJournal of Medical Microbiology
Department/SchoolTasmanian School of Medicine
PublisherPathological Society of Great Britain and Ireland
Place of publicationUK