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Characterization of a type IV bundle-forming pilus (SFP) from a gastroenteritis-associated strain of Aeromonas veronii biovar sobria
journal contributionposted on 2023-05-16, 10:18 authored by Kirov, SM, Sanderson, K
The colonization mechanisms of enteropathogenic Aeromonas strains are poorly characterized, but recent studies indicate that some filamentous structures are intestinal adhesins. This study describes the purification and characterization of a long, flexible pilus from a gastroenteritis-associated strain of Aeromonas veronii biovar sobria. SDS-PAGE analysis (various conditions) of pill preparations yielded a pilin protein band of ~21 kDa. Its N-terminal amino acid sequence was unambiguous and homologous with those of type IV pilins. Immunogold electron microscopy with rabbit antisera produced against this pi[in protein (SFP) decorated single pill and rope-like bundles of pill on the bacterial surface. These were seen more frequently on strains grown at 22Â°C compared with 37Â°C and in liquid rather than on solid medium. SFP was not detected on any of 104 strains of Aeromonas (different species and sources) from our culture collection, although morphologically similar structures were seen on a number of these strains. This finding and differences among other published amino acid sequences show that Aeromonas type IV pill are antigenically diverse. Bundle-forming type IV 'class B' pill are important in the virulence of other enteropathogenic bacteria. The N-terminal amino acid sequence of the Aeromonas SFP, however, showed closer homology to the type IV 'class A' pilins. Studies are in progress to investigate the role of SFP in Aeromonas virulence.
Publication titleMicrobial Pathogenesis
Department/SchoolTasmanian School of Medicine
Place of publicationLondon