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Characterization of latent and active forms of cartilage proteinases produced by normal immature rabbit articular-cartilage in tissue-culture

journal contribution
posted on 2023-05-17, 08:19 authored by Cartwright, EC, Campbell, IK, Margaret BritzMargaret Britz, Sandy, JD, Lowther, DA
Cultured tissue slices from normal immature rabbit articular cartilage released latent neutral metal‐loproteinases into serum‐free medium. On activation with 4‐aminophenylmercuric acetate, these metalloproteinases could degrade collagen, proteoglycan, and gelatin. Also produced were an acid proteinase with the properties of cathepsin D and an inhibitor of the neutral metalloproteinases. The appearance of both the proteinases and the inhibitor in the culture medium could be prevented by incubation of cultures with cycloheximide. The active and latent forms of the proteinases were characterized using Ultrogel AcA 54 chromatography. Copyright © 1983 American College of Rheumatology

History

Publication title

Arthritis and Rheumatism

Volume

26

Issue

8

Pagination

984-993

ISSN

0004-3591

Department/School

College Office - College of Sciences and Engineering

Publisher

Wiley-Liss

Place of publication

Div John Wiley & Sons Inc, 605 Third Ave, New York, USA, Ny, 10158-0012

Repository Status

  • Restricted

Socio-economic Objectives

Clinical health not elsewhere classified

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