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Chemical Characterization of Exopolysaccharides from Antarctic Marine Bacteria

journal contribution
posted on 2023-05-16, 14:53 authored by Mancuso Nichols, CA, Lardiere, SG, John BowmanJohn Bowman, Nichols, PD, Gibson, JAE, Guezennec, J
Exopolysaccharides (EPS) may have an important role in the Antarctic marine environment, possibly acting as ligands for trace metal nutrients such as iron or providing cryoprotection for growth at low temperature and high salinity. Ten bacterial strains, isolated from Southern Ocean particulate material or from sea ice, were characterized. Whole cell fatty acid profiles and 16S rRNA gene sequences showed that the isolates included representatives of the genera Pseudoalteromonas, Shewanella, Polaribacter, and Flavobacterium as well as one strain, which constituted a new bacterial genus in the family Flavobacteriaceae. The isolates are, therefore, members of the "Gammaproteobacteria" and Cytophaga-Flexibacter-Bacteroides, the taxonomic groups that have been shown to dominate polar sea ice and seawater microbial communities. Exopolysaccharides produced by Antarctic isolates were characterized. Chemical composition and molecular weight data revealed that these EPS were very diverse, even among six closely related Pseudoalteromonas isolates. Most of the EPS contained charged uronic acid residues; several also contained sulfate groups. Some strain produced unusually large polymers (molecular weight up to 5.7 MDa) including one strain in which EPS synthesis is stimulated by low temperature. This study represents a first step in the understanding of the role of bacterial EPS in the Antarctic marine environment. © Springer Science+Business Media, Inc. 2005.

History

Publication title

Microbial Ecology

Volume

49

Issue

4

Pagination

578-589

ISSN

0095-3628

Department/School

Tasmanian Institute of Agriculture (TIA)

Publisher

Springer Verlag

Place of publication

New York, USA

Repository Status

  • Restricted

Socio-economic Objectives

Oceanic processes (excl. in the Antarctic and Southern Ocean)

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