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Children with nut allergies have impaired gene expression of Toll-like receptors pathway
Methods: In a cross-sectional study, samples from 80 children with and without food allergies were analysed for gene expression, DNA methylation and a range of immune factors relating to TLR pathways. TLR2, TLR4, CD14, IL5, IL13 and vitamin D were explored.
Results: The importance of these immune factors appeared to vary between the different types of food allergies. Expression of TLR2 (P < .001), TLR4 (P = .014) and CD14 (P = .028) varied significantly between children with no food allergy, allergy to nuts and peanuts, and allergy to eggs. DNA methylation in the promoter regions of these genes had a significant association with gene expression. These trends persisted when subjects were stratified by nut allergy vs no nut allergy. Furthermore, TLR2 (P = .001) and CD14 (P = .007) expressions were significantly lower in children with food allergies when compared to those without.
Conclusion: Gene expression of TLR pathway genes was directly related to food allergy type, and DNA methylation had an indirect effect. TLR2 pathways are of significant interest in nut allergies.
Publication titlePediatric Allergy and Immunology
Department/SchoolMenzies Institute for Medical Research
Place of publication35 Norre Sogade, Po Box 2148, Copenhagen, Denmark, Dk-1016
Rights statementCopyright 2020 EAACI and John Wiley and Sons A/S