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Compatible solute accumulation and stress-mitigating effects in barley genotypes contrasting in their salt tolerance

journal contribution
posted on 2023-05-16, 20:59 authored by Chen, Z, Tracey Cuin, Meixue ZhouMeixue Zhou, Twomey, A, Bodapati, PN, Sergey ShabalaSergey Shabala
The accumulation of compatible solutes is often regarded as a basic strategy for the protection and survival of plants under abiotic stress conditions, including both salinity and oxidative stress. In this work, a possible causal link between the ability of contrasting barley genotypes to accumulate/synthesize compatible solutes and their salinity stress tolerance was investigated. The impact of H2O2 (one of the components of salt stress) on K+ flux (a measure of stress 'severity') and the mitigating effects of glycine betaine and proline on NaCl-induced K+ efflux were found to be significantly higher in salt-sensitive barley genotypes. At the same time, a 2-fold higher accumulation of leaf and root proline and leaf glycine betaine was found in salt-sensitive cultivars. The total amino acid content was also less affected by salinity in salt-tolerant cultivars. In these, potassium was found to be the main contributor to cytoplasmic osmolality, while in salt-sensitive genotypes, glycine betaine and proline contributed substantially to cell osmolality, compensating for reduced cytosolic K +. Significant negative correlations (r= -0.89 and -0.94) were observed between Na+-induced K+ efflux (an indicator of salt tolerance) and leaf glycine betaine and proline. These results indicate that hyperaccumulation of known major compatible solutes in barley does not appear to play a major role in salt-tolerance, but rather, may be a symptom of salt-susceptibility. © 2007 The Author(s).


Grains Research & Development Corporation


Publication title

Journal of Experimental Biology










Tasmanian Institute of Agriculture (TIA)


Oxford University Press

Place of publication

United Kingdom

Repository Status

  • Restricted

Socio-economic Objectives


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