Compatible solutes reduce ROS-induced potassium efflux in Arabidopsis roots
journal contribution
posted on 2023-05-16, 20:27 authored by Tracey Cuin, Sergey ShabalaSergey ShabalaReactive oxygen species (ROS) are known to be primarily responsible for the impairment of cellular function under numerous abiotic and biotic stress conditions. In this paper, using non-invasive microelectrode ion flux measuring (MIFE) system, we show that the application of a hydroxyl radical (OH •)-generating Cu 2+/ascorbate (Cu/a) mixture to Arabidopsis roots results in a massive, dose-dependent efflux of K + from epidermal cells in the elongation zone. Pharmacological experiments suggest that both outward-rectifying K + channels and non-selective cation channels (NSCCs) mediate such effluxes. Low (5 mM) concentrations of compatible solutes (glycine betaine, proline, mannitol, trehalose or myo-inositol) significantly reduces OH •-induced K + efflux, similar to our previous reports for NaCl-induced K + efflux. Importantly, a significant reduction in K + efflux was found using osmolytes with no reported free radical scavenging activity, as well as those for which a role in free radical scavenging has been demonstrated. This indicates that compatible solutes must play other (regulatory) roles, in addition to free radical scavenging, in mitigating the damaging effects of oxidative stress. © 2007 The Authors.
History
Publication title
Plant Cell and EnvironmentVolume
30Issue
7Pagination
875-885ISSN
0140-7791Department/School
Tasmanian Institute of Agriculture (TIA)Publisher
Blackwell Publishing LtdPlace of publication
GermanyRepository Status
- Restricted
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