Cyclic mononucleotides modulate potassium and calcium flux responses to H2O2 in Arabidopsis roots
journal contributionposted on 2023-05-18, 01:57 authored by Ordonez, NM, Marondedze, C, Thomas, L, Pasqualini, S, Svetlana ShabalaSvetlana Shabala, Sergey ShabalaSergey Shabala, Gehring, C
Cyclic mononucleotides are messengers in plant stress responses. Here we show that hydrogen peroxide (H2O2) induces rapid net K+-efflux and Ca2+-influx in Arabidopsis roots. Pre-treatment with either 10 μM cAMP or cGMP for 1 or 24 h does significantly reduce net K+-leakage and Ca2+-influx, and in the case of the K+-fluxes, the cell permeant cyclic mononucleotides are more effective. We also examined the effect of 10 μM of the cell permeant 8-Br-cGMP on the Arabidopsis microsomal proteome and noted a specific increase in proteins with a role in stress responses and ion transport, suggesting that cGMP is sufficient to directly and/or indirectly induce complex adaptive changes to cellular stresses induced by H2O2.
Publication titleFEBS Letters
Department/SchoolTasmanian Institute of Agriculture (TIA)
PublisherElsevier Science Bv
Place of publicationPo Box 211, Amsterdam, Netherlands, 1000 Ae
Rights statementLicensed under Creative Commons Attribution-NonCommercial-NoDerivs 3.0 Unported (CC BY-NC-ND 3.0) http://creativecommons.org/licenses/by-nc-nd/3.0/